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For rapid and efficient extraction of plasmid DNA from bacterial cells.
The method is based on a combination of alkaline lysis and RNase treatment to obtain a clear lysate with minimal genomic DNA and RNA contamination. The bound plasmid DNA is washed and finally eluted by adding a buffer. The test kit contains the I-Blue Lysis Buffer, an optional colour indicator. The use of this indicator prevents common handling errors that impair efficient cell lysis and neutralization.
Specifications | I-Blue Mini Plasmid Kit // I-Blue Midi Plasmid Kit |
Method: | Spin column // Anion-Exchange |
Sample size: | 1 - 7 ml // 50 - 100 ml high-copy plasmid / 100 - 150 ml low-copy plasmid |
Binding capacity: | 50 µg // 500 µg |
Fragment size: | 1 - 15 kb // 1 - 20 kb |
Typical yield: | up to 50 µg // 200 - 500 µg |
Operation Time: | <15 min. // <80 min. |