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Plasmid Purification-Kits I-Blue Mini / Midi
For rapid and efficient extraction of plasmid DNA from bacterial cells.
The method is based on a combination of alkaline lysis and RNase treatment to obtain a clear lysate with minimal genomic DNA and RNA contamination. The bound plasmid DNA is washed and finally eluted by adding a buffer. The test kit contains the I-Blue Lysis Buffer, an optional colour indicator. The use of this indicator prevents common handling errors that impair efficient cell lysis and neutralization.
- Fast extraction time
- High yield
- Prevention of handling errors during cell lysis and neutralisation by I-Blue colour indicator
| Specifications | I-Blue Mini Plasmid Kit // I-Blue Midi Plasmid Kit |
| Method: | Spin column // Anion-Exchange |
| Sample size: | 1 - 7 ml // 50 - 100 ml high-copy plasmid / 100 - 150 ml low-copy plasmid |
| Binding capacity: | 50 µg // 500 µg |
| Fragment size: | 1 - 15 kb // 1 - 20 kb |
| Typical yield: | up to 50 µg // 200 - 500 µg |
| Operation Time: | <15 min. // <80 min. |
Plasmid Purification-Kits I-Blue Mini / Midi
Plasmid purification Kits
Nucleic acid purification
DNA purification kits
DNA plasmid extraction
Plasmid purification Kits
Nucleic acid purification
DNA purification kits
DNA plasmid extraction
Plasmid purification Kits
Nucleic acid purification
DNA purification kits
DNA plasmid extraction
Plasmid purification Kits
Nucleic acid purification
DNA purification kits
DNA plasmid extraction