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Home > Life Science > Genomics > DNA and RNA isolation > Gel/PCR DNA Fragment Extraction Kit

Gel/PCR DNA Fragment Extraction Kit

The test kit was developed to obtain or concentrate DNA fragments from agarose gels, PCR or other enzymatic processes.
The agarose gel is dissolved, the enzymes are denatured and the DNA fragments will bind to the glass fibre matrix in the spin column. Wash buffers (containing ethanol) are used to remove contaminants and a low salt elution buffer is used to recover the purified DNA fragments. Recoveries are 90 - 95 % for PCR clean-up. With this kit, PCR purification and gel extraction procedures can be performed, so that a second test kit is not necessary.

High recovery rate
Short process time
One test kit for two methods

Specifications
Sample size:	up to 300 mg of agarose gel / up to 100 µl of PCR product
Binding capacity:	10 µg DNA
Fragment size:	<10 kb
Typical yield:	80-90% gel extraction / 90-95% PCR clean-up
Operation Time:	<20 min.