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Calibrate Your ProDSS Water Quality Meter like a PRO!


The Ultimate Guide for the YSI ProDSS instrument provides helpful insights, instructions and troubleshooting tips for calibrating your ProDSS water quality meter.


YSI-ProDSS-calibration-guide.jpgThe guide includes a Calibration Worksheet to help you track and document all your calibrations and the performance of your sensors. For accurate calibration results, be sure to follow the calibration procedures in the manual of the meter and to check that your calibration solutions are fresh.

What You'll Find in the Guide?


Temperature | Conductivity | pH | ORP | DO | Turbidity | Depth |

Ammonium | Nitrate | Chloride


You will also find instructions on how to:
Keep reading or download the easy printable guide to get all the tips and start calibrating your ProDSS like a pro!

Don't have a ProDSS yet? Be sure to read our post on the 7 Things You Need to Know about the ProDSS or download the newly issued brochure with all the main highlights about the ProDSS.

Should you need any further information about the ProDSS water quality meter, please contact our Sales Team.


Temperature Calibration Tips


Since accurate temperature compensation is required for all parameters (with the exception of the turbidity and TSS), temperature accuracy should be verified and recorded each time the ProDSS is calibrated. For this reason, before calibrating any other ProDSS sensor, verify the temperature sensor is reading accurately by comparing it to a traceable thermometer.

Be sure to consider the specification tolerances of both the ProDSS temperature sensor and the thermometer when comparing the measurements.

Temperature Troubleshooting Tips


It’s essential to make sure the ProDSS temperature sensor is clean and reading properly. To clean the sensor, use the conductivity cleaning brush – or a simple toothbrush. Cleaning with a little warm water and mild detergent should do the trick. This sensor does not require calibration.

If you are using a ProDSS 4 port cable (that uses a replaceable sensor) and your temperature sensor is not reading accurately even after cleaning, remove the conductivity/temperature sensor and check the port and connector for moisture or any damage.


Conductivity Calibration Tips


The conductivity calibration should be verified every day the instrument is used.

  1. Calibrating any of these parameters: conductivity, specific conductance and salinity, will simultaneously calibrate the others. YSI recommends calibrating specific conductance (temperature compensated conductivity) for greatest ease and accuracy
  2. Make sure the conductivity sensor is clean and dry before performing a specific conductance calibration
  3. Always use fresh, traceable conductivity calibration solution when calibrating the conductivity sensor
    1. The shelf life of conductivity solution is one month after being opened. This is due to potential changes in the value of the solution caused by evaporation which can occur after opening the bottle. Writing the open date on the bottle is a good way to keep track if the calibration solution is still good.
    2. Never calibrate with a conductivity solution that is less than 1.0 mS/cm. You are setting the slope on a linear device so a good, strong conductivity signal will give you the best performance. Use 1.0 mS/cm for fresh water, 10 mS/cm for brackish to estuarine water and 50 mS/cm for salt water. Please note that 1.0 mS (millisiemens) = 1000 uS (microsiemens).
  4. Pre-rinse the cal cup and sensors with a small amount of calibration standard or rinse standard and discard
  5. Cover the conductivity sensor up to the top vent holes with the calibration solution. If the sensor is not totally covered in the solution, the instrument will read approximately half the expected value
    1. If using a ProDSS 4 port cable, the top vent hole is located on the side of the combination conductivity/temperature sensor. Filling the ProDSS calibration cup to line 2 (i.e. the top line) when the cup is empty will ensure the vent hole is covered.
    2. OR If using the ODO/CT assembly, ensure the vent holes at the top of the sensor are completely immersed and the solution level is at least 1/2 inch higher than these top vent holes.
  6. After placing the sensor into the solution, gently move the sensor up and down to remove any air bubbles that may be trapped in the conductivity sensor
  7. If calibrating Specific Conductance, enter the value of the conductivity solution as it is listed for 25 °C. Make sure you are entering the correct units. 1 mS = 1,000 uS
Questionable calibration: if you receive this warning message, stop the calibration. Select ‘No’ and check your steps to see what could be causing the questionable results. Typical causes for this error message include: incorrect entries (entering 1000 uS/cm instead of 1.0 mS/cm), air bubbles trapped in the sensor, not enough solution to cover the vent holes, calibrating in conductivity instead of specific conductance, dirty conductivity electrodes, and/or bad calibration solution.

Please note that your conductivity readings (and your DO mg/L readings) will not be correct if you accept a questionable calibration. After accepting a good calibration, navigate to the GLP file and check the conductivity cell constant for the calibration. The cell constant should be 5.0 to 6.0 for highest accuracy (4.9 to 5.9 on ODO/CT probe and cable assemblies). However, 4.5 to 6.5 is the acceptable range (4.4 to 6.4 on ODO/CT cables).

Conductivity Troubleshooting Tips


If you get an error message during calibration, be sure that you are:

  • Entering the correct calibration value (1 mS/cm = 1000 uS/cm)
  • Calibrating in Specific Conductance mode
  • Using enough solution to cover the vent holes on the sensor
  • Dislodging any air bubbles that could be trapped in the sensor
  • Using a fresh, traceable conductivity calibration solution
Still getting the error, even after following these recommendations? Check the conductivity sensor to make sure it is clean. A clean conductivity sensor should read less than 1 uS/cm in dry air. If your sensor is dry and giving you a reading higher than 1 uS/cm in air, it should be cleaned.

Any significant jump or change in the conductivity cell constant from one calibration to the next usually indicates a problem with the calibration and/or sensor. If your calibration standard is good and the calibration process is correct, then your sensor may need to be cleaned.

Cleaning the Conductivity Sensor

You should clean the openings that allow sample access to the conductivity electrodes quite regularly using the small cleaning brush included with each new conductivity sensor and cable. Dip the brush in clean water and insert it into each hole 10 to 12 times.

If deposits have formed on the electrodes, you can use a mild detergent (laboratory grade soap or bathroom foaming tile cleaner) with the brush. Rinse thoroughly with clean water, then check the response and accuracy of the conductivity sensor with calibration solution.

Cables with User-Replaceable Sensors

If using a 4 port cable and your conductivity sensor is not calibrating or is reading > 1 uS/cm in dry air after being cleaned, remove the conductivity/temperature sensor from the cable and inspect the sensor port and connector for damage or moisture. Please follow the steps to Cleaning a Sensor Port if needed.

Cables with Integral (i.e. built-in) Sensors

If your conductivity sensor is not calibrating or is reading > 1 uS/cm in dry air after performing a sensor cleaning, contact your local YSI Representative or a YSI Authorised Service Centre. Lab Unlimited is an authorised Service Centre for the whole of Ireland. Contact us through our Service Portal or at technical.support@labunlimited.com.


pH Calibration Tips


The pH calibration should be verified every day the instrument is used. However, a new pH sensor may be capable of holding its calibration for several days.


  1. The pH sensor can be calibrated with up to three calibration points
  2. Calibration can be accomplished in any buffer order
  3. pH 7 buffer should be used regardless of how many calibration points you use
  4. In most cases, a two-point calibration is sufficient (4 and 7 or 7 and 10). You can bracket the expected in-situ pH values. Use a three-point calibration with 4, 7 and 10 if the in-situ pH values are unknown or if you expect the in-situ values to be on both sides of the pH scale
  5. Use a bit of pH buffer to rinse the sensors and the cal cup. Fill the cup so that the pH sensor tip and the temperature sensor are submerged in buffer
  6. Calibration values will not have to be entered if using a USA (4, 7, 10) or a NIST (4.01, 6.86, 9.18) buffer set, as the ProDSS will automatically recognize these buffers and will compensate the calibration value for temperature. The buffer set can be changed in the pH Sensor Setup menu
  7. Record the pH millivolts for each calibration point. These are the acceptable mV outputs for each buffer:
    • pH 7 mV value = 0 mV +/- 50 mV
    • pH 4 mV value = +165 to +180 from pH 7 buffer mV value
    • pH 10 mV value = -165 to -180 from pH 7 buffer mV value
    • A value of +50 or -50 mVs in buffer 7 does not indicate a bad sensor. The mV span between pH 4 and 7 and 7 and 10 mV values should be ≈ 165 to 180 mV. 177 is the ideal distance. The slope can be 55 to 60 mV per pH unit with an ideal of 59 mV per pH unit. If the mV span between pH 4 and 7 or 7 and 10 drops below 160, clean the sensor and recalibrate
  8. Wait for the measurement to stabilise in each buffer and then press Enter to accept each calibration point
  9. Make sure to use a small amount of the next buffer to rinse the sensor and cal cup between calibration points
  10. If you want to finish calibration after 1 or 2 points, select Finish Calibration. Otherwise, the calibration will automatically be completed after accepting the third point in a 3 point calibration
Stop calibration if you receive a questionable calibration message. If you accept this calibration, your pH readings will be wrong. Simply select ‘No’ and check your steps to see what is causing the questionable results. Typical causes for this error message include: incorrect buffer set selected, a dirty sensor, or bad buffer solution.

After accepting a good calibration, navigate to the GLP file and check the pH Slope and Slope % of ideal. A good slope should be between 55 and 60 mVs while the ideal is 59 mV. If the slope drops below 53, the sensor should be reconditioned and recalibrated.

pH Troubleshooting Tips


Depending on maintenance, storage and usage, a typical working life for pH sensors is approximately 12-24 months. Good storage and proper maintenance usually extend the sensor’s life. Getting an error message during a pH calibration?
Be sure to check:

  • If the pH buffers are good and not expired
  • The correct buffer set is enabled
  • Are the glass bulb or the electrode body damaged
  • The sensor module is installed correctly
If you continue to get error messages during calibration, if you notice a slow response in the field, or if it’s taking longer than 90 seconds to stabilise the pH buffer, make sure to clean and recondition the sensor as explained below.

Cleaning and Reconditioning the pH, ORP or pH/ORP Sensor

If the pH or pH/ORP sensor has dried out or has been stored in distilled or deionized water for a long time, be sure to soak the sensor in buffer 4 overnight to try and restore functionality. Cleaning the sensor is usually required if/when the sensor’s response slows and if deposits or contaminants appear on the glass and/or platinum surfaces. The cleaning can be chemical and/or mechanical.

It is easier to clean the sensor if it’s removed from the cable. Start by dampen a soft clean cloth, lens cleaning tissue or cotton swab to remove all foreign material from the glass bulb and/or platinum button. Then use a moistened cotton swab to carefully remove any material that may be blocking the reference electrode junction of the sensor.

CAUTION: When using a cotton swab, be careful NOT to wedge the swab between the guard and the glass sensor. If necessary, remove cotton from the swab tip, so that the cotton can reach all parts of the sensor tip without stress. You can also use a pipe cleaner for this, if convenient. If good pH and/or ORP response is not restored, perform the following additional procedure:

  • Soak the sensor for 10-15 minutes in clean water containing a few drops of commercial dishwashing liquid
  • Rinse the sensor in clean water, wipe with a cotton swab moistened with clean water, and then re-rinse with clean water.

If good pH and/or ORP response is still not restored or if hard deposits have built up on the electrode, perform the following additional procedure:

  • Soak the sensor for ~3 minutes in one molar (1 M) hydrochloric acid (HCl). This reagent can be purchased from most lab supply distributors. Be sure to follow the safety instructions included with the acid. You can also use vinegar, but it will need a longer period of soaking
  • Rinse the sensor in clean water, wipe with a cotton swab moistened with clean water (not DI water), and then re-rinse with clean water. To be certain that all traces of the acid are removed from the sensor crevices, soak the sensor in clean tap water for about an hour with occasional stirring

If biological contamination of the reference junction is suspected or if good response is not restored by the above procedures, perform the following additional cleaning steps:

CAUTION: Do not mix the acid from the previous step with the chlorine bleach in the following step. A toxic gaseous product can form from the reaction between the acid and the chlorine bleach. Be certain to copiously rinse the sink and drain system of acid after its disposal and before the disposal of chlorine bleach.

  • Soak the sensor for approximately 1 hour in a 1:1 dilution of commercially available chlorine bleach
  • Rinse the sensor with clean water and then soak for at least 1 hour in clean tap water with occasional stirring to remove residual bleach from the junction. (If possible, soak the sensor for more than 1 hour to be sure all traces of chlorine bleach are removed.) Then re-rinse the sensor with clean water and retest
  • Prior to reinstalling the sensor, dry the port and sensor connector with compressed air. If you suspect port contamination, follow the instructions on how to Clean a Sensor Port before reinstalling the sensor
If your pH sensor is still not calibrating after performing a sensor cleaning, contact your local YSI Representative or a YSI Authorised Service Centre. Lab Unlimited is an authorised Service Centre for the whole of Ireland. Contact us through our Service Portal.




ORP (Oxidation Reduction Potential) Calibration Tips


The ORP calibration should be verified every day the instrument is used. However, a new ORP sensor may be capable of holding its calibration for several days.

  1. If using a pH/ORP combination sensor, calibrate pH first to ensure it is working
  2. Use ORP calibration solution to rinse the sensors and cal cup. Fill the cup so that the ORP sensor tip and the temperature sensor are submerged in solution
  3. If using YSI Zobell calibration solution, the ProDSS will automatically adjust the calibration value based on temperature. Otherwise, the Calibration value can be manually adjusted
  4. Wait for the readings to stabilise and then press Enter to accept the calibration
  5. Do not continue with calibration if you get a questionable calibration message. If you accept this calibration your ORP readings will be incorrect. Simply select ‘No’ and investigate what could be causing these results – usually a dirty sensor or a bad calibration solution

ORP Troubleshooting Tips


Depending on maintenance, storage and usage, a typical working life for ORP sensors is approximately 12-24 months. Good storage and proper maintenance usually extend the sensor’s life.

Clean and recondition the sensor if the sensor exhibits a slow response in Zobell solution, i.e. it takes more than 90 seconds to stabilise when placed in Zobell.

  • Be sure to check that the ORP calibration solution is good and not expired.
  • If you continue to get error messages during calibration, or if the sensor exhibits a slow response in Zobell solution (i.e. it takes more than 90 seconds to stabilise when placed in Zobell), clean and recondition the sensor as per the instructions in the pH Troubleshooting section above
  • If you suspect port contamination, follow the instructions for Cleaning a Sensor Port before reinstalling the sensor
  • If you continue to have problems, you can check the offset of the ORP sensor by performing a factory reset to the ORP sensor.

After resetting the sensor, compare the ORP mV readings in Zobell solution to the calibration value. The difference between values should be less than 100 mVs. If the difference is 80 mVs or higher, consider replacing the sensor as it is nearing the end of its life span.


Dissolved Oxygen Calibration Tips


The dissolved oxygen sensor should be calibrated every day the instrument is used. It is not necessary to calibrate in both % and mg/L or ppm. Calibrating in % will simultaneously calibrate mg/L and ppm and vice versa.

  1. The ProDSS optical DO sensor can be calibrated in air-saturated water, water-saturated air or against a Winkler Titration. You can perform a 1 or 2 point DO calibration. A 2 point calibration includes 1 point in a zero oxygen environment and the 2nd point at full saturation
  2. For both ease of use and accuracy, YSI recommends that you perform a 1 point calibration in water-saturated air
  3. Make sure that there is a good optical DO sensor cap installed. The cap should not be scratched or excessively dirty. Caps should be changed as needed (15-18 month expected life for caps with a 1 year warranty)
  4. To perform a 1 point calibration in water-saturated air, place the sensor in a 100% humid environment. This can be accomplished several ways:
    1. For the ProDSS 4 port cables, place a small amount of water in the calibration/storage cup and place it over the sensors and sensor guard. Partially tighten the locking ring on the calibration cup to the bulkhead. The goal is to have air exchange between inside and outside the calibration cup.
    2. For the ProDSS ODO/CT or ProODO cables, moisten the sponge in the grey calibration sleeve with a small amount of clean water and place it over the sensor guard.
  5. As bacterial growth may consume oxygen and interfere with calibration, make sure the sponge and calibration sleeve/cup are cleaned, that the sensor is in air, not water, and that there are not any water droplets on the sensor cap or temperature sensor
  6. After entering the % calibration mode, wait approximately 5 to 10 minutes for the storage container to become completely saturated
  7. Salinity affects the ability of water to hold oxygen and is used by the instrument to calculate DO mg/L (ppm). The Salinity value displayed near the top of the DO calibration screen is either the Salinity value as measured by the conductivity sensor in use or the salinity correction value entered in the Sensor menu. If using a conductivity sensor, make sure that it is calibrated and reading correctly in order to obtain accurate DO mg/L (ppm) measurements. If you are not using a conductivity sensor, the Salinity correction value should be the salinity of the water you will be testing. Press the Probe key, highlight Salinity, and press Enter to modify this setting if necessary. The salinity of fresh water is typically 0-0.5 ppt and seawater is typically 35 ppt.

If you get a questionable calibration message, do not continue with the process. If you accept a questionable calibration, your DO readings will not be correct. Select ‘No’ and check your steps to see what is causing the bad results. What could cause a calibration error message is usually a dirty and/or bad sensor cap or a sensor that needs reconditioned.


DO Troubleshooting Tips

  • Verify that the ProDSS barometer is reading accurately. If this is not the case, then your calibration will not be correct. The DO % Saturation calibration uses the instrument’s barometric pressure reading for the DO % calibration. The barometer should be reading true barometric pressure. If you suspect the barometer is not reading correctly, calibrate it and then recalibrate the DO sensor. Laboratory barometer readings are usually “true” (uncorrected) values of air pressure and can be used “as is” for barometer calibration. Weather service readings are usually not “true”, i.e., they are corrected to sea level, and therefore cannot be used until they are “uncorrected”. An approximate formula for this “uncorrection” is: True BP in mmHg = Corrected BP in mmHg – [2.5 * (Local Altitude in ft. above sea level/100)]
  • Clean the ODO sensor cap and rehydrate it if needed
  • If you have changed the sensor cap, ensure the sensor cap coefficients have correctly been entered. These can be seen under Sensor Setup on the handheld, or within KorDSS
  • If you suspect port contamination, remove the sensor and follow the instructions to Cleaning a Sensor Port

If you continue to have trouble calibrating the DO sensor, contact your local YSI Representative or a YSI Authorised Service Centre. Lab Unlimited is an authorised Service Centre for the whole of Ireland. Contact us through our Service Portal.

ODO Sensor Cap Replacement

If the sensor cap is damaged or cracked it should be replaced. Also, once a year you should replace the sensor cap (for those with a 1 year warranty), but the cap may last longer. When you are replacing the sensor cap, make sure you are looking at the right sensor instructions, as the instructions for replacing the sensor cap on ProDSS ODO sensors are different from integral (i.e. built-in) ODO sensors on ODO/CT and ProODO cable assemblies.

Each replacement ODO sensor cap is shipped in a humidified container and the package should not be opened until immediately before sensor cap replacement. The instruction sheet shipped with the replacement ODO sensor cap includes the calibration coefficients specific to your sensor cap. Make sure to save this instruction sheet in case you need to reload the calibration coefficients. These coefficients must be entered whenever the sensor cap has been replaced.

Coefficients can be entered using the ProDSS handheld (under ODO Sensor Setup) or KorDSS (under the Instrument and Sensors tab).

Cleaning the ODO Sensor Cap

After some time, your sensor can be blocked with an organic or inorganic film build-up, which could affect the dissolved oxygen measurements. To avoid this, you should keep your sensor cap clean. To do this, softly wipe away any fouling with a damped (in water) lens cleaning tissue.

Caution: Do not use organic solvents to clean the sensor cap. Using an organic solvent to clean the sensor cap may cause permanent damage to the cap. For example, alcohol will dissolve the outer paint layer and other organic solvents will likely dissolve the dye in the cap.

Rehydrating the ODO Sensor Cap

The ODO sensor should always be stored in a wet or water-saturated air environment to avoid sensor drift. If left to dry for longer than 8 hours, it must be rehydrated by soaking the ODO sensor cap in warm (room temperature) tap water for approximately 24 hours. After the soak, be sure to calibrate the sensor.


Turbidity Calibration Tips


The turbidity calibration should be verified every day the instrument is used. However, the turbidity sensor is very stable and may hold its calibration for several weeks.

  1. For proper calibration, you must use standards that have been prepared according to details in Standard Methods for the Treatment of Water and Wastewater (Section 2130 B). Standards from other vendors are NOT approved, and their use will likely result in a bad calibration and incorrect field readings. Acceptable standards include:
    1. AMCO-AEPA standards prepared specifically for the ProDSS turbidity sensor manufactured by YSI (i.e. YSI turbidity standards)
    2. Formazin prepared according to Standard Methods, especially for calibration points greater than 1010
    3. Dilutions of 4000 FNU (NTU) formazin concentrate purchased from Hach
    4. Hach StablCal™ standards in various FNU (NTU) denominations
  2. It is important to use the same type of standard for all calibration points (i.e. do not mix formazin and AMCO-AEPA standard for different points in a multi-point calibration)
  3. The ProDSS turbidity sensor can be calibrated by using up to three calibration points by using the following limits:
    1. 1st calibration point: 0-1 FNU (NTU) (see Preventing Negative Turbidity Readings below)
    2. 2nd calibration point: 5-200 FNU (NTU)
    3. 3rd calibration point: 400-4200 FNU (NTU)
  4. DI water can be used for the first calibration point (see Preventing Negative Turbidity Readings below)
  5. The ProDSS calibration cup and sensor guard must be used (and correctly installed!) when calibrating. The sensor guard must be installed when taking any measurements
  6. The sensor guard has a metal bottom that is painted black to eliminate any stray light reflection. Make sure the inside surface (i.e. the surface that faces the sensor tip) is not significantly scratched. Also ensure the sensor guard and calibration cup are free of any reflective material
  7. Pour standard slowly down the side of the calibration container so you do not aerate the sample. This will reduce the possibility of air bubbles becoming trapped on the surface of the sensor
  8. Slowly place the turbidity sensor into the calibration cup when the cup is tilted at a 45 degree angle, as this will help prevent air bubbles from being caught on the sensor surface
  9. Wait for the turbidity measurement to stabilise in each standard and then press Enter to accept each calibration point
  10. Use a small amount of the next standard between calibration points to rinse the sensor and cal cup
  11. If you want to finish calibration after accepting 1 or 2 points, select Finish Calibration. Otherwise, the calibration will automatically be completed after accepting the third point in a 3 point calibration

Do not accept the calibration if you get a questionable calibration message. If you accept it, your turbidity readings will not be correct. Select ‘No’ and investigate could be causing the issue. Typical causes for this error message include a dirty sensor or bad standard solution.


Turbidity Troubleshooting Tips


The ProDSS turbidity sensor has a 2 year warranty and there are no replaceable components (e.g. no optical sensor cap). Proper storage and maintenance will help extend the sensor's life. If you get error messages during a turbidity calibration, check if:

  • Standard solutions are good and not expired
  • The calibration environment (e.g. calibration cup, sensor guard, and sensors) is clean. See Preventing Negative Turbidity Readings if you have any issues with negative turbidity readings
  • There should not be any reflective material on the sensor guard and calibration cup. The metal sensor guard bottom (inside; faces the sensors) should be free of any scratches
  • If you suspect port contamination, remove the sensor and follow the instructions to Cleaning a Sensor Port

If you continue to have trouble calibrating the turbidity sensor, contact your local YSI Representative or a YSI Authorised Service Centre. Lab Unlimited is an authorised Service Centre for the whole of Ireland. Contact us through our Service Portal or at technical.support@labunlimited.com.

Cleaning the Turbidity Sensor

To clean the sensing window, you should use a non-abrasive, lint-free cloth and if necessary, use mild soapy water. This should be done carefully to prevent scratches.

Preventing Negative Turbidity Readings

A negative turbidity reading is almost always connected to the 'zero' standard. Despite best practices, you’ll find that sometimes it is impossible to clean the sensors, calibration cup, and sensor guard to a point where the 'zero' standard will not be contaminated by some small amount. A new instrument can contaminate a zero standard to ~0.1 FNU, even in a lab environment.

Also, a cleaned, but used, ProDSS sensor, calibration cup and sensor guard can contaminate a zero standard to almost 1.0 FNU. As an example, if a Calibration Value of zero is entered, but the actual reading in the 'zero' standard is 0.6 FNU, then a ProDSS turbidity sensor in a 0.3 FNU environment will display a measurement of -0.3 FNU. Since the 'zero' calibration environment may not be 0 FNU due to contaminated standard, dirty sensors, dirty calibration cup, and/or dirty sensor guard, a Calibration Value from 0 to 1 FNU can be entered.

The following tips can help eliminate negative turbidity readings:

  • Use a calibration cup and sensor guard exclusively for calibration. Calibration cups and sensor guards can easily become contaminated over time, especially if the instrument is used to measure in dirty samples and/or field conditions
  • In cases where the equipment is properly cleaned and serviced, the level of contamination of the zero turbidity standard is quite small. Typically, the average contaminant level ranges from 0.2 to 0.8 NTU. Knowing this, you can pick a number between these points (0.5) and enter this as the first calibration point
  • Calibrating turbidity is best done in a lab environment; calibrations in the field can result in errors
  • The only true way to determine if your zero standard is being contaminated is to analyse the zero solution with a laboratory turbidimeter

Depth Calibration Tips


The depth calibration is very easy to perform and should be completed every time the instrument is used to take depth measurements. Simple follow these steps below:
  1. Input a Depth Offset, Altitude, or Latitude under Sensor Setup if desired. Entering a value for these is not required to complete a calibration
    1. Depth offset: Depth offset can be used if referencing water elevation against a known datum. If a depth offset is entered (in meters), the output value will shift by the value of the offset. The most common offset entered is 0.272 meters, as this is the distance from the depth sensor on 4 port cables to the sensor tips.
    2. Altitude and Latitude: To compensate for atmospheric pressure based on elevation and gravitational pull, enter the local altitude in meters relative to sea level and latitude in degrees where the ProDSS is sampling. This will ensure highest accuracy, although the altitude and latitude effects are relatively small. Varying altitudes cause approximately 90 mm change from sea level to 8000 m. A 100 m change causes 1.08 mm of change to the readings. Varying latitudes cause a 200 mm change in depth from equator to pole.
  2. Ensure the depth sensor is cleaned, in air and not immersed in any solution
  3. For highest accuracy, keep the bulkhead still and in one position while calibrating. The holes on the side of the depth sensor should not be covered
  4. The Calibration Value will be set at zero even if a depth offset is entered. There is no need to change this as long as you're calibrating in air
  5. Wait for the depth measurement to stabilise and then press Enter to accept the calibration. Only a 1 point calibration can be completed
Getting a warning message stating that the calibration is questionable? Do not continue with the calibration. If you accept this calibration, your depth readings will be incorrect. Select ‘No’ and investigate what is causing the questionable results. Typical causes of a calibration error message include dirty ports on the side of the depth sensor, not waiting for stable measurements before accepting the calibration, moisture in the depth ports, and/or covering the depth ports with your hand during calibration.

Depth Troubleshooting Tips


The YSI ProDSS depth sensor measures virtually vented depth. This type of measurement allows for real time compensation for atmospheric pressure using the instrument's internal barometer. A major advantage to this type of depth sensor is there is no vented cable, tube or desiccant to worry about. Some troubleshooting tips include:

  • During calibration, make sure that the torts on the side of the depth sensor are not covered and are free of any debris. These ports can be cleaned with the syringe included with the maintenance kit. To clean the ports, fill the syringe with clean water and gently force water into one of the ports. Flush until clean water flows from the opposite depth port
  • A sensor guard weight installed at the end of the sensor guard can help keep the bulkhead stable when sampling at depth. Up to 5 lbs of YSI stackable sensor guard weights can be installed
  • Enable Vertical Position under Depth Display to view the real-time position of the depth sensor in the water column. This is helpful in profiling applications to ensure the depth sensor is lowered to the desired depth without waiting for the depth data to stabilise.

Ammonium Calibration Tips


The ammonium sensor should only be used in fresh water (salinity < 2 ppt) and to depths of 55 feet (17 meters) of water. It should be calibrated every day the instrument is used. Ammonia is calculated from the ammonium, temperature and pH readings. pH greatly affects the ammonia calculation, so for highest accuracy in the ammonia calculation, be sure to use a pH sensor in conjunction with an ammonium sensor during measurements.

If a pH sensor is not in use, the instrument will assume the sample is neutral (pH 7) for the calculation. Be sure to follow these steps for calibration:

  1. Exposure to the high ionic content of pH buffers can cause a significant, but temporary, drift in the ammonium sensor. Therefore, if calibrating a pH sensor, either:
    1. Remove the ammonium sensor from the cable bulkhead and plug the port. After pH calibration is complete, reinstall the ammonium sensor and proceed with its calibration with no stabilisation delay.
    2. OR Calibrate pH first, immersing both sensors in the pH buffers. After calibrating pH, place the sensors in 100 mg/L ammonium standard and monitor the reading. Usually, the reading starts low and may take a while to reach a stable value. When it does, proceed with the calibration. This may take several hours.
  2. The ammonium sensor can be calibrated with up to three calibration points. For highest accuracy, perform a two point calibration with 1 and 100 mg/L standards within 10 °C of your sample temperature
  3. Rinse the sensors and cal cup with a small amount of ammonium solution (1 mg/L for the first point and 100 mg/L for the second point). Fill the cup so that the ammonium sensor tip and the temperature sensor are submerged in solution. Ensure the conductivity sensor is also submerged in the calibration solution. The salinity reading from the conductivity sensor is used in the algorithm for the ammonium measurement
  4. After entering the calibration screen, change the calibration value if necessary
  5. Record the NH4 millivolts for each calibration point. The acceptable mV outputs for each calibration solution are shown below.
    • NH4 1 mg/L = 0 mV +/- 20 mV (new sensor only)
    • NH4 100 mg/L = 90 to 130 mV from 1 mg/L mV value
    • The mV span between 1 mg/L and 100 mg/L values should be ≈ 90 to 130 mV. The slope should be 45 to 65 mV per decade.
  6. Wait for the ammonium and temperature readings to stabilise in each calibration solution and then press Enter to accept each calibration point
  7. Be sure to use a small amount of the next standard to rinse the sensor and cal cup between calibration points
  8. After pressing Enter to accept the second calibration point, highlight Finish Calibration and press Enter to complete the calibration. Otherwise, you can continue calibrating with a third calibration point (see the ProDSS User Manual for more information on a chilled third calibration point)

If you receive a warning message stating that the calibration is questionable, do not continue with the calibration. Instead, select ‘No’ and investigate what is causing the questionable results. If you accept a questionable calibration, your ammonium and ammonia readings will be incorrect. Typical causes for this error message include a dirty sensor or bad standard solution.


Preparing Ammonium Calibration Solution

We recommend using YSI calibration solutions whenever possible. However, qualified users can save cost by following the following recipes for 1 and 100 mg/L standards. Other concentrations can be made by altering the amount of ammonium chloride. All other ingredient concentrations should remain unchanged.

It is important to note that some of these chemicals are hazardous and therefore, the standards should only be prepared by qualified chemists in laboratories where proper safety precautions are possible. It is the responsibility of the user to obtain and study the MSDS for each chemical and to follow the required instructions regarding handling and disposal of these materials.

You will need: solid Ammonium Chloride or a certified 100 mg/L NH4+-N standard solution from a supplier, Lithium Acetate Dihydrate, concentrated hydrochloric acid, high purity water, a good quality analytical balance, a 1000 mL volumetric flask, accurate volumetric measuring devices for 100 mL and 10 mL of solution, and a 1000 mL glass or plastic storage vessels.

(Caution: Hydrochloric acid is highly corrosive and toxic and should therefore be handled with extreme care in a well-ventilated fume hood. The user could also add the equivalent amount of a less-hazardous, more dilute sample of the acid if preferred.)

100 mg/L Standard:

Accurately weigh 0.3817 g of ammonium chloride and transfer quantitatively into a 1000 mL volumetric flask. Add 2.6 g of lithium acetate dihydrate to the flask. Add approximately 500 mL of distilled or deionized water to the flask, swirl to dissolve all the reagents and then dilute to the volumetric mark with distilled or deionized water. Mix well by repeated inversion and then transfer the 100 mg/L standard to a storage bottle. Add 3 drops of concentrated hydrochloric acid to the bottle, then seal and agitate to assure homogeneity. Alternatively, 100 mL of certified 100 mg/L NH4 +-N standard can be used in place of the solid ammonium chloride.

1 mg/L Standard:

Accurately measure 10.0 mL of the above 100 mg/L standard solution into a 1000 mL volumetric flask. Add 2.6 g of lithium acetate dihydrate to the flask. Add approximately 500 mL of distilled or deionized water, swirl to dissolve the solid reagents and then dilute to the volumetric mark with water. Mix well by repeated inversion and then transfer the 1 mg/L standard to a storage bottle. Add 3 drops of concentrated hydrochloric acid to the bottle, then seal and agitate to assure homogeneity.

Ammonium Troubleshooting Tips


Depending on usage, maintenance and storage, the typical working life for ammonium sensors is approximately 3-6 months. A good keep up and storage of the sensors usually extend their working life. If you get error messages during an ammonium calibration, check the following:
  • Ensure the ammonium solutions are good and not expired
  • Clean the sensor
  • If you continue to get error messages during calibration, soak the sensor in 100 mg/L ammonium standard for several hours or overnight
  • If you suspect port contamination, follow the instructions follow the instructions to Cleaning a Sensor Port
If you continue to have trouble calibrating the ammonium sensor, contact your local YSI Representative or a YSI Authorised Service Centre. Lab Unlimited is an authorised Service Centre for the whole of Ireland. Contact us through our Service Portal or at technical.support@labunlimited.com.

Cleaning the Ammonium Sensor

The ammonium sensor uses a PVC membrane. As always, when handling a sensor, care should be taken to avoid damaging the membrane. After extensive use, the membranes may become coated with a deposit or scoured with fine scratches which may cause a slow or reduced response (low slope) or unstable readings. Deposits may be removed with a fine jet of deionized water or rinsing in alcohol followed by soaking in 100 mg/L ammonium calibration standard. The sensor may require soaking in the high ammonium calibration solution to recover its performance. Soak in 100 mg/L for several hours or overnight.

Nitrate Calibration Tips


The nitrate sensor should only be used in fresh water (salinity < 2 ppt) and to depths of 55 feet (17 meters) of water. The nitrate sensor should be calibrated every day the instrument is used. Be sure to follow these steps for calibration:

  1. Exposure to the high ionic content of pH buffers can cause a significant, but temporary, drift in the nitrate sensor. Therefore, if calibrating a pH sensor, either:
    1. Remove the nitrate sensor from the cable bulkhead and plug the port. After pH calibration is complete, reinstall the nitrate sensor and proceed with its calibration with no stabilisation delay.
    2. OR Calibrate pH first, immersing both sensors in the pH buffers. After calibrating pH, place the sensors in 100 mg/L nitrate standard and monitor the reading. Usually, the reading starts low and may take a while to reach a stable value. When it does, proceed with the calibration. This may take several hours.
  2. The nitrate sensor can be calibrated with up to three calibration points. For highest accuracy, perform a two point calibration with 1 and 100 mg/L standards within 10 °C of your sample temperature
  3. Rinse the sensors and cal cup with a small amount of nitrate solution (1 mg/L for the first point and 100 mg/L for the second point). Fill the cup so that the nitrate sensor tip and the temperature sensor are submerged in solution. Ensure the conductivity sensor is also submerged in the calibration solution. The salinity reading from the conductivity sensor is used in the algorithm for the nitrate measurement
  4. After entering the calibration screen, change the calibration value if necessary
  5. Record the NO3- millivolts for each calibration point. The acceptable mV outputs for each calibration solution are shown below.
    • NO3- 1 mg/L = 200 mV +/- 20 mV (new sensor only)
      • NO3- 100 mg/L = -90 to -130 mV from 1 mg/L mV value
    • The mV span between 1 mg/L and 100 mg/L values should be ≈ 90 to 130 mV. The slope should be -45 to -65 mV per decade
  6. Wait for the nitrate and temperature readings to stabilise in each calibration solution and then press Enter to accept each calibration point
  7. Rinse the sensor and cal cup between calibration points with a small amount of the next standard
  8. After pressing Enter to accept the second calibration point, highlight Finish Calibration and press Enter to complete the calibration. Otherwise, you can continue calibrating with a third calibration point (see the ProDSS User Manual for more information on a chilled third calibration point)

If you receive a warning message stating that the calibration is questionable, do not continue with the calibration. Instead, select ‘No’ and investigate what is causing the questionable results. If you accept a questionable calibration, your nitrate readings will be erroneous. Typical causes for this error message include a dirty sensor or bad standard solution.


Preparing Nitrate Calibration Solution

We recommend using YSI calibration solutions whenever possible. However, qualified users can save cost by following the following recipes for 1 and 100 mg/L nitrate standards. Other concentrations can be made by altering the amount of potassium nitrate. All other concentrations should remain unchanged.

It is important to note that some of these chemicals are hazardous and therefore, the standards should only be prepared by qualified chemists in laboratories where proper safety precautions are possible. It is the responsibility of the user to obtain and study the MSDS for each chemical and to follow the required instructions with regards to handling and disposal of these materials.

You will need: Solid Potassium Nitrate or a certified 1000 mg/L NO3--N from a supplier, Magnesium Sulphate, high purity water, good quality analytical balance, 1000 mL volumetric flask, accurate volumetric measuring devices for 100 mL, 10 mL and 1 mL of solution, and 1000 mL glass or plastic storage vessels.

100 mg/L standard:

Accurately weigh 0.7222 g of anhydrous potassium nitrate and transfer quantitatively into a 1000 mL volumetric flask. Add 1.0 g of anhydrous magnesium sulphate to the flask. Add approximately 500 mL of water to the flask, swirl to dissolve all of the reagents, and then dilute to the volumetric mark with distilled or deionized water. Mix well by repeated inversion and then transfer the 100 mg/L standard to a storage bottle. Rinse the flask extensively with water prior to its use in the preparation of the 1 mg/L standard. Alternatively, 100 mL of certified 1000 mg/L NO3--N standard can be used in place of the solid potassium nitrate.

1 mg/L standard:

Accurately measure 10.0 mL of the above 100 mg/L standard solution into a 1000 mL volumetric flask. Add 1.0 g of anhydrous magnesium sulphate to the flask. Add approximately 500 mL of distilled or deionized water, swirl to dissolve the solid reagents, and then dilute to the volumetric mark with water. Mix well by repeated inversion and then transfer the 1 mg/L standard to a storage bottle. Recipes are given for 1 and 100 mg/L. Other concentrations can be made by altering the amount of potassium nitrate. All other concentrations should remain unchanged.


Nitrate Troubleshooting Tips


Typical working life for nitrate sensors is approximately 3-6 months depending on use, storage and maintenance. Proper storage and maintenance generally extends the sensor’s working life. If you get error messages during a nitrate calibration, check the following:

  • Ensure the nitrate solutions are good and not expired
  • Clean the sensor
  • If you continue to get error messages during calibration, soak the sensor in 100 mg/L nitrate standard for several hours or overnight
  • If you suspect port contamination, follow the instructions in the follow the instructions to Cleaning a Sensor Port

If you continue to have trouble calibrating the nitrate sensor, contact your local YSI Representative or a YSI Authorised Service Centre. Lab Unlimited is an authorised Service Centre for the whole of Ireland. Contact us through our Service Portal or at technical.support@labunlimited.com.


Cleaning and Reconditioning the Nitrate Sensor

The nitrate sensor uses a PVC membrane. As always, when handling a sensor, care should be taken to avoid damaging the membrane. After extensive use the membranes may become coated with a deposit or scoured with fine scratches which may cause a slow or reduced response (low slope) or unstable readings.

Deposits may be removed with a fine jet of deionized water or rinsing in alcohol followed by soaking in 100 mg/L nitrate calibration standard. The sensor may require soaking in the high nitrate calibration solution to recover its performance. Soak in 100 mg/L for several hours or overnight.


Chloride Calibration Tips


The chloride sensor should only be used in fresh water (salinity < 2 ppt) and to depths of 55 feet (17 meters) of water. This sensor should be calibrated every day the instrument is used. Be sure to follow these steps for calibration:

  1. Exposure to the high ionic content of pH buffers can cause a significant, but temporary, drift in the chloride sensor. Therefore, if calibrating a pH sensor, either:
    1. Remove the chloride sensor from the cable bulkhead and plug the port. After pH calibration is complete, reinstall the chloride sensor and proceed with its calibration with no stabilisation delay.
    2. OR Calibrate pH first, immersing both sensors in the pH buffers. After calibrating pH, place the sensors in 1,000 mg/L chloride standard and monitor the reading. Usually, the reading starts low and may take a while to reach a stable value. When it does, proceed with the calibration. This may take several hours.
  2. The chloride sensor can be calibrated with up to three calibration points. For highest accuracy, perform a two point calibration with 10 and 1000 mg/L standards within 10 °C of your sample temperature
  3. Rinse the sensors and cal cup with a small amount of chloride solution (10 mg/L for the first point and 1,000 mg/L for the second point). Fill the cup so that the chloride sensor tip and the temperature sensor are submerged in solution. Ensure the conductivity sensor is also submerged in the calibration solution. The salinity reading from the conductivity sensor is used in the algorithm for the chloride measurement
  4. After entering the calibration screen, change the calibration value if necessary
  5. Record the Cl millivolts for each calibration point. The acceptable mV outputs for each calibration solution are shown below.
    • Cl 10 mg/L = 225 mV +/- 20 mV (new sensor only)
    • Cl 1,000 mg/L = -80 to -130 mV from 10 mg/L mV value
    • The mV span between 10 mg/L and 1000 mg/L values should be ≈ 80 to 130 mV. The slope should be -40 to -65 mV per decade
  6. Wait for the chloride and temperature readings to stabilise in each calibration solution and then press Enter to accept each calibration point
  7. Use a small amount of the next buffer to rinse the sensor and cal cup between calibration points
  8. After pressing Enter to accept the second calibration point, highlight Finish Calibration and press Enter to complete the calibration. Otherwise, you can continue calibrating with a third calibration point (see the ProDSS User Manual for more information on a chilled third calibration point)

If you receive a questionable calibration message, do not continue with the calibration. If you accept this your chloride readings will not be correct. Instead, select ‘No’ and investigate what is causing the questionable results. Typical causes for this error message include a dirty sensor or bad standard solution.


Preparing Chloride Solution

The following recipes are provided for preparation of 10 and 1000 mg/L chloride reagents. It is important to note that some of the chemicals required for these solutions could be hazardous under some conditions. It is the responsibility of the user to obtain and study the MSDS for each chemical and to follow the required instructions regarding handling and disposal of these chemicals.

You will need: Solid sodium chloride or a certified 1000 mg/L chloride solution from a supplier, magnesium sulphate, high purity water, a good quality analytical balance, 1000 mL volumetric flask, an accurate 10 mL measuring device, and 1000 mL glass or plastic storage vessels.

1000 mg/L standard:

Accurately weigh 1.655 grams of anhydrous sodium chloride and transfer into a 1000 mL volumetric flask. Add 0.5 grams of anhydrous magnesium sulphate to the flask. Add 500 mL of distilled or deionized water to the flask, swirl to dissolve all of the reagents, and then dilute to the volumetric mark with water. Mix well by repeated inversion and then transfer the 1000 mg/L standard to a storage bottle. Rinse the flask extensively with water prior to its use in the preparation of the 10 mg/L standard. Alternatively, simply add 0.5 grams of magnesium sulphate to a litre of a 1000 mg/L chloride standard from a certified supplier.

10 mg/L standard:

Accurately measure 10 mL of the above 1000 mg/L standard solution into a 1000 mL volumetric flask. Add 0.5 grams of anhydrous magnesium sulphate to the flask. Add 500 mL of distilled or deionized water, swirl to dissolve the solid reagents, and then dilute to the volumetric mark with water. Mix well by repeated inversion and then transfer the 10 mg/L standard to a storage bottle.


Chloride Troubleshooting Tips


Typical working life for chloride sensors is approximately 3-6 months depending on use, storage and maintenance. This can be extended if proper storage and maintenance occur. If you get error messages during a chloride calibration, check the following:

  • Ensure the chloride solutions are good and not expired
  • Clean the sensor
  • If you continue to get error messages during calibration, soak the sensor in 1000 mg/L chloride standard for several hours or overnight
  • If you suspect port contamination, follow the instructions follow the instructions to Cleaning a Sensor Port

If you continue to have trouble calibrating the chloride sensor, contact your local YSI Representative or a YSI Authorised Service Centre. Lab Unlimited is an authorised Service Centre for the whole of Ireland. Contact us through our Service Portal or at technical.support@labunlimited.com.


Cleaning and Reconditioning the Chloride Sensor

The chloride sensor is considered a pellet membrane ISE. As always, when handling sensors, care should be taken to avoid damaging the membrane. This sensor can be regenerated by washing with alcohol and/or gently polishing with fine emery paper in a circular motion to remove any deposits or discoloration, then thoroughly washing with deionized water to remove any debris.

The sensor may require soaking in the high chloride calibration solution to recover its performance. Soak in 1000 mg/L for several hours or overnight.


Installing and Uninstalling Sensors


General Precautions: When installing, removing or replacing sensors it is essential that the entire sensor connector and cable connector are dry, as this will prevent water from entering any ports. After removing the sensor, the next step would be to check the connector inside the port for moisture.

If any moisture is present, use compressed air to completely dry the connector or place directly in front of a steady flow of fresh air. If you suspect port contamination, follow the port cleaning procedures listed under Cleaning a Sensor Port.

Remove sensors with the sensor tips facing the ground to help prevent water from entering the port upon removal.

The instrument utilises o-rings as seals to prevent water from entering the sensor ports. When the sensors are removed, the o-rings that provide the seal should be carefully inspected for contamination (e.g. debris, grit, etc.) and cleaned if necessary.

If no dirt or damage to the o-rings is evident, wipe the o-rings with a lint free cloth or lens cloth to remove the old o-ring grease. Then, lightly apply new o-ring grease (provided in the maintenance kit) to the o-rings without removing them from their groove.

If there is any indication of damage, the o-ring should be replaced with an identical o-ring. At the time of o-ring replacement, the entire o-ring assembly should be cleaned. Do not over-grease the o-rings. The purpose of the o-ring grease it to keep the o-ring in good condition. Excess grease may collect grit particles that can compromise the seal and also cause the waterproofing capabilities of the o-ring to diminish, potentially causing leaks. If excess grease is present, remove it using a lens cloth or lint-free cloth.

To remove the o-rings: Use a small, flat-bladed screwdriver or similar blunt-tipped tool to remove the o-ring from its groove. Do not use a sharp object to remove the o-rings. Using a sharp object could damage the o-ring groove which would allow water to enter the port resulting in permanent damage to the port and sensor. Check the o-ring and the groove for any excess grease or contamination.

If contamination is evident, clean the o-ring and the portion of the titanium sensor where the o-ring fits with lens cleaning tissue or equivalent lint-free cloth. Alcohol can be used to clean the titanium sensor, but use only water and mild detergent on the o-ring itself.

Using alcohol on o-rings may cause a loss of elasticity and may promote cracking. Also, inspect the o-rings for nicks and imperfections. Before re-installing the o-rings, make sure to use a clean workspace, clean hands, and avoid contact with anything that may leave fibres on the o-ring or grooves. Even a very small amount of contamination (hair, grit, etc.) may cause a leak.

To re-install the o-rings: Place a small amount of o-ring grease between your thumb and index finger. Draw the o-ring through the grease while pressing the fingers together to place a very light covering of grease to the o-ring. Place the o-ring into its groove making sure that it does not twist or roll. Do not excessively stretch the o-ring during installation.

Use your grease-coated finger to once again lightly go over the mating surface of the o-ring. Do not over-grease the o-rings. The excess grease may collect grit particles that can compromise the seal. Excess grease can also cause the waterproofing capabilities of the o-ring to diminish, potentially causing leaks. If excess grease is present, remove it using a lens cloth or lint-free cloth.


Cleaning a Sensor Port


If you suspect port contamination, you can clean the port on the cable by filling the port with Isopropyl Alcohol for 30 seconds and then dumping it out. It is very important to allow the port to air dry completely or blow it out with compressed air. Installing a sensor into a port that is not completely dry is likely to cause incorrect or inconsistent readings. If the connector is corroded, contact your local YSI Representative or a YSI Authorised Service Centre. Lab Unlimited is an authorised Service Centre for the whole of Ireland. Contact us through our Service Portal or at technical.support@labunlimited.com.



Verifying Sensor Accuracy and Calibration


Sensor accuracy and calibration can be verified by immersing a sensor into calibration solution or YSI Confidence Solution®. Compare the readings on the ProDSS display to the value of the solution. If the readings have drifted more than the accuracy specification of the sensor, perform a calibration before taking field measurements. You can use the YSI Confidence Solution to check the accuracy and calibration of the conductivity, pH and ORP sensors.

However, to maintain the highest accuracy of the instrument, it should not be used to perform a calibration.


Resetting a Sensor to Factory Default


If you need to reset the instrument to factory calibration values, all you have to do is press the Cal key, highlight Restore Default Cal and press Enter. Highlight the parameter you wish to reset to default and press Enter. Next, you will be asked to confirm the operation. Highlight Yes and press Enter to confirm.

Lab Unlimited is an authorised Service Centre for the whole of Ireland. You can contact us at technical.support@labunlimited.com or through our Service Portal, where units can be returned to us for inspection, calibration or repair.






























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