Lactose Sulfite Broth Base is a selective medium recommended by ISO 7937 for the confirmation of Clostridium perfringens based on lactose fermentation and production of hydrogen sulphide. The nutrient base provides optimal conditions for the development of Clostridium. Casein peptone provides nitrogen, vitamins, minerals, and amino acids essential for growth. Yeast extract is the source of vitamins, particularly of the B-group, and lactose is a complex carbohydrate energy source. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Cysteine hydrochloride is the reducing agent. Colonies producing hydrogen sulphide are characterized by a blackening due to the reaction of Sodium bisulphite and the Ferric ammonium citrate salt. The containers showing a blackening and abundant formation of gas in the Durham tube (at least 1/10 of the volume) indicate the presence of C. perfringens.
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