To view pricing in Euro(€) visit: www.labunlimited.com X
Your basket is empty
Home > Life Science > Molecular Cell Biology > DNA Cloning > TA Cloning Kits > Canvax pSpark® TA Done DNA Cloning Kit C0021
Image Unavailable
Enlarge

Canvax pSpark® TA Done DNA Cloning Kit C0021

PART NO: CX-C0021
Price: £158.36

pSpark® TA Done DNA Cloning Kit, for efficient, stable & easy cloning of blended and non-proofreading pcr fragments with ecori and noti flanking the insertion site

pSpark® TA Done is efficient, stable and easy-to-use DNA cloning vector based on an improved TA technology that offers all of the advantages of pSpark® TA with the added convenience of recognition sites for EcoRI and NotI flanking the insertion site. Thus, several options exist to remove the desired insert DNA with a single restriction digestion.

Main Features:

Convenient: recognition sites for EcoRI and NotI flanking the insertion site. Flexible: allows removing the desired insert DNA with other restriction digestion. Efficient: more than 600 white positive colonies expected under optimal conditions. Stable: without cloning bias due to transcription of toxic genes. Easy-to-use: eliminates screening of recombinants due to its low background (4%). Fast protocol: ligation time from 60 minutes to overnight. Compatible: with direct cloning of PCR products. Great versatility: compatible with any competent cell, primer design or particular proofreading DNA Polymerase. Cost avoidance: avoids primer phosphorylation. Risk-free: product covered by Canvax Quality 100% Guarantee.

Includes: Includes for 20 rxn: - 20 µL pSpark® TA Done (50 ng/µL) - 20 µL T4 DNA Ligase (5U/Weiss) - 200 µL T4 DNA Ligase Buffer (5x) - 5 µL Insert Control 1 kb (20 ng/µL)

Applications: Cloning of non-proofreading PCR fragments. Production of ssDNA. Blue/white screening for recombinants. In vitro transcription from T7/SP6 dual-opposed promoters.

Format: 20 rxn
Shipping conditions: Gel Pack
Storage Conditions: -20 ºC
ManufacturerCanvax
Manufacturer P/nC0021
Powered by Prospect

We use cookies to give you the best possible experience on our site. By continuing to use the site you agree to our use of cookies. Dismiss | More Information X