Canvax First Strand cDNA Synthesis Kit PR008

First Strand cDNA Synthesis Kit, efficient & sensitive complete system for first strand cdna synthesis from rna templates

First-Strand cDNA Synthesis Kit is an Efficient &amp, Sensitive System for First strand cDNA Synthesis from RNA templates. It includes s all the necessary components to synthesize first-strand cDNA, except the template RNA (total RNA or mRNA). First-Strand cDNA Synthesis Kit features two optimized mixes, RT Enzyme Mix and RT Reaction Mix. RT Enzyme Mix combines Reverse Transcriptase and RNase Inhibitor, while RT Reaction Mix contains dNTPs, oligo(dT)18 primers, random hexamers, MgCl2 and an optimized buffer. The synthesized single-stranded cDNA is suitable for real-time quantitative PCR applications. The Kit has been formulated to provide high yields of full-length cDNA product and to increase sensitivity in RT-qPCR. Starting material can range from 10 pg to 5 µg of total RNA.

Main Features:

Convenient: the Kit includes all the components needed. Highly Sensitive: full length cDNA starting at 10 pg - 5 µg of total RNA, due a novel RNase H treatment. Full-length first strand cDNA up to 10kb. Optimized to increase sensitivity in RT-qPCR and RT-PCR assays. Optimal reaction temperature: 50 °C Avoids RNA template degradation.

Includes: Includes for 100 rxn: · 2 x 100µL RT Enzyme Mix · 2 x 500µL RT Reaction Mix (2x) · 100 µL RNase H (E. coli) · 2 x 1 mL Nuclease-free H2O

Applications: • First Strand cDNA Synthesis for RT-PCR and RT-qPCR. • Construction of full length cDNA Libraries. • RNA Analysis.