Canvax pColiExpress™ II LIC Cloning & Expression Kit BE005-S

pColiExpress™ II LIC Cloning & Expression Kit, for a highly efficient & versatile protein expression (lic technology based) in e. coli from pcr fragment cloned

pColiExpress™ II LIC Cloning &amp, Expression Kit is a highly efficient, versatile and fast system of DNA cloning vectors for protein expression in E. coli. All family systems are based in directional ligase-independent-cloning (LIC) technology, a rapid procedure that provides high-cloning efficiencies and the fast production of a large quantity of any desired protein. It contains all elements required for expression of large quantities of any desired protein by T7 RNA Polymerase Inducible System and a His6 tag at the COOH end of the protein that allows the purification. The vector has also an f1 origin of replication, an Ampicillin resistance cassette, and the pBR322 origin of replication.

Main Features:

Ready-to-use vectors. Highly efficient cloning system. Special design that allows the cell to house larger numbers of copies than other plasmids with ori pBR322. Linearized vector: ready for ligation with your PCR amplified with the recommended primers. Minimum background: lower than 1%. Time-saving protocol: avoids any step required after PCR. Higher protein expression than other suppliers. Cost avoidance: avoids the use of expensive phosphorylated primers. Versatility: cloning of PCR fragments amplified with any type of DNA Polymerase. Risk-free: product covered by Canvax Quality 100% Guarantee.

Includes: Includes for 20 rxn: - 20 µL pColiExpress™ linearized vector (50 ng/µL) - 50 µL LIC Enzyme Buffer (10x) - 40 µL LIC Enzyme (10 U/µL) - 10 µL Insert Control DNA (30 ng/µL) - 5 µL pColiExpress™ Control - Uncut vector (50 ng/µL)

Applications: Cloning of PCR fragments for subsequent expression of proteins in E. coli. Cloning of PCR fragments with Glue Enzyme. Expression of proteins under the control of the T7 promoter. Protein expression in BL21 (DE3) or BL21 (DE3) (pLys).