Canvax pMBL™ T-Vector DNA Cloning Kit C0030

pMBL™ T-Vector DNA Cloning Kit, for a efficient, convenient & fast cloning of dna fragments with a overhangs

pMBL™ T-Vector DNA Cloning Kit is an efficient, convenient and fast system for the cloning of PCR products. The vector is prepared by cutting pMBL™ T-Vector with EcoRV and adding a 3´ terminal thymidine to both ends. These single 3´-T overhangs at the insertion site greatly improve the efficiency of ligation of a PCR product into the plasmids by preventing recircularization of the vector and providing a compatible overhang for PCR products generated by certain thermostable polymerases such as Horse-Power™ Taq DNA Polymerase. These Polymerases often add a single deoxyadenosine, in a template-independent fashion, to the 3´-ends of the amplified fragments.

Main Features:

Highly efficient: greater than 90% white colonies in a transformation with supplied insert control. Proven performance: more than 1,000 recombinant colonies expected in optimal conditions. Fast and easy protocol: results from 15 min protocol. Optimized: improve efficiency of ligation of a PCR product into the plasmid. Compatible: overhang for ligation of PCR products preventing recircularization of the vector. Designed by cutting the vector with EcoRV and adding a 3´ terminal thymidine to both ends. Risk-free: product covered by Canvax Quality 100% Guarantee.

Includes: Includes for 20 rxn: - 20 µL pMBL™ T-Vector (50 ng/µL) - 20 µL T4 DNA Ligase (5U/Weiss) - 100 µL T4 DNA Ligase Buffer (10x) - 5 µL Insert Control 600 bp (30 ng/µL)

Applications: Cloning of PCR fragments into DNA Cloning vector. Blue/white screening for recombinants.