Canvax T4 DNA Ligase CL005

T4 DNA Ligase, for highly efficient, accurate & easy ligation of dna insert into plasmid vectors from 5 minutes

T4 DNA Ligase is highly efficient, accurate and rapid enzyme designed for an efficient ligation of cohesive and blunt ended DNA insert into plasmid vectors in 5-15 minutes. It is based on the combination of T4 DNA Ligase with a Premium 5x Ligation Buffer. The ligation reaction mixture is used directly for bacterial transformation using conventional transformation procedures. It enables sticky end or blunt end DNA ligation in only 5 minutes at Room Temperature.

Main Features:

Recombinant: isolated from E. coli. Highly efficient: 3,500 white colonies and 10 blue colonies expected under optimal conditions. Fast and easy protocol: get 24 % of efficiency at 5 minutes and 75 % at 15 minutes. Powerful: allows cloning insert amounts < 1 ng/kb. Versatile: enables sticky end or blunt end DNA Ligation. Risk-free: product covered by Canvax Quality 100% Guarantee.

Includes: Includes for 300 U: - 60 µL T4 DNA Ligase (5 U Weiss/µL) - 250 µL T4 DNA Ligase Buffer (5x)

Applications: Routine cloning procedure: blunt and cohesive double stranded DNA cloning. TA cloning. Joining of adaptors with blunt or cohesive ends.