08452 30 40 30
Canvax HotBegan™ Hot Start Taq DNA Polymerase P0028
PART NO: CX-P0028
Price: £63.95
HotBegan™ Hot Start Taq DNA Polymerase, for a specific, highly efficient & reliable amplifications of dna up 160 fg
HotBegan™ Hot Start Taq DNA Polymerase is a specific, highly efficient and sensitive HotStart DNA Polymerase designed to minimize unspecific amplification, improving PCR specificity. It is a Horse-Power™ Taq DNA Polymerase bound to a proprietary antibody that blocks polymerase activity until a denaturation step occurs. The heat-labile antibodies are rapidly inactivated by raising the temperature (4 minutes at 95-97 °C). This prevents or minimizes primer-dimer and non-specific products. Like Horse-Power™ Taq DNA Polymerase, the enzyme has 5’?3’ polymerase activity and a weak 5’?3’ exonuclease activity but no 3’?5’ exonuclease activity (proofreading). Before enzyme activation none of the enzyme activities are detectable.
Main Features:
High specificity: minimizes unspecific amplification. Efficient: prevents or minimizes primer-dimer and nonspecific products. Great sensitivity: amplifies from femptograms of DNA targets. Inactive: at Room Temperature. Optimized: adds extra nucleotides (preferentially adenine) without template at 3´ends leaving 3´overhangs PCR fragments. Powerful: amplification of targets up to 5 kb. Convenient: available in kits and MasterMix solution. Risk-free: product covered by Canvax Quality 100% Guarantee.
Includes: Includes for 500 U: - 100 µL HotBegan™ Hot Start Taq DNA Polymerase (5 U/µL) - 25 mM MgCl2 (1.5 mL) - 1.5 mL Buffer (10x)  
Applications: qPCR. PCR and RT-PCR. Genotyping with Taqman Probes. PCR fragments amplification for TA or GC cloning. Amplification from a limited DNA template or low copy number genes.
HotBegan™ Hot Start Taq DNA Polymerase is a specific, highly efficient and sensitive HotStart DNA Polymerase designed to minimize unspecific amplification, improving PCR specificity. It is a Horse-Power™ Taq DNA Polymerase bound to a proprietary antibody that blocks polymerase activity until a denaturation step occurs. The heat-labile antibodies are rapidly inactivated by raising the temperature (4 minutes at 95-97 °C). This prevents or minimizes primer-dimer and non-specific products. Like Horse-Power™ Taq DNA Polymerase, the enzyme has 5’?3’ polymerase activity and a weak 5’?3’ exonuclease activity but no 3’?5’ exonuclease activity (proofreading). Before enzyme activation none of the enzyme activities are detectable.
Main Features:
High specificity: minimizes unspecific amplification. Efficient: prevents or minimizes primer-dimer and nonspecific products. Great sensitivity: amplifies from femptograms of DNA targets. Inactive: at Room Temperature. Optimized: adds extra nucleotides (preferentially adenine) without template at 3´ends leaving 3´overhangs PCR fragments. Powerful: amplification of targets up to 5 kb. Convenient: available in kits and MasterMix solution. Risk-free: product covered by Canvax Quality 100% Guarantee.
Includes: Includes for 500 U: - 100 µL HotBegan™ Hot Start Taq DNA Polymerase (5 U/µL) - 25 mM MgCl2 (1.5 mL) - 1.5 mL Buffer (10x)  
Applications: qPCR. PCR and RT-PCR. Genotyping with Taqman Probes. PCR fragments amplification for TA or GC cloning. Amplification from a limited DNA template or low copy number genes.
| Format: 5 U/µL, 500 U Shipping conditions: Gel Pack Storage Conditions: -20 ºC | |
| Manufacturer | Canvax |
| Manufacturer P/n | P0028 |